Additional details for
Samuel Adediran, Eric Larsen, Jim Hatcher, and Rao Adibhatla (Biology)
"The roles of phospholipases on reduced phosphatidylcholine (PC) levels after oxygen and glucose deprivation in PC12 cells treated with Tricyclodecan-9-yl-xanthogenate (D609)"
The Kentucky Academy of Science, November 9-11 , December 31, 1969
Loss of phosphatidylcholine (PC) is a metabolic response to stroke/ischemia. PC loss compromises the integrity of cell membrane and disrupts cell ionic gradient. In vivo models of stroke have shown PC loss to result from increase in the activity of three phospholipases namely, PC-PLC, PLA2 and PC-PLD. However, in PC12 cells, used as in vitro model for ischemia, activity of PLA2 does not increase after oxygen and glucose deprivation (OGD). Only the activities of PC-PLC and PC-PLD increase. In order to reduce PC hydrolysis and maintain PC levels, D609 was used to inhibit PC-PLC in OGD PC12 cells. D609, however, increased PC hydrolysis, cell death, and the levels of free fatty acids in PC12 cells after OGD. In this study, we investigated whether D609 increased PC hydrolysis by increasing the activity of PLA2 or the activity of PC-PLD. We found that D609 did not increase PLA2 activity, and that the increase in free fatty acids levels did not result from elevated PLD activity. Also, D609 induced apoptosis in PC12 cells after OGD and triggered the externalization of phosphatidylserine in the membrane. Lastly, D609 was found to increase the release of cytochrome C from the mitochondria.
"The roles of phospholipases on reduced phosphatidylcholine (PC) levels after oxygen and glucose deprivation in PC12 cells treated with Tricyclodecan-9-yl-xanthogenate (D609)"
The Kentucky Academy of Science, November 9-11 , December 31, 1969
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