Dr. Miranda (Gibson) Beam
Visiting Assistant Professor of Organic Chemistry
Visiting Assistant Professor of Organic Chemistry
B.S. Chemistry, Berea College, 2003
Ph.D. Pharmaceutical Sciences, University of Kentucky, 2008
miranda_beam@berea.edu
Ph.D. Pharmaceutical Sciences, University of Kentucky, 2008
miranda_beam@berea.edu
Work Experience
- Visiting Professor of Organic Chemistry Aug. 2008-present Berea College, Berea, KY 40404 Teach General and Organic Chemistry
- Analytical Chemist Chempharma, Richmond, KY Jan. 2006-August 2008 Rodney Eisenberg, Ph.D., Director Performed contract NMR experiments and assisted in background research
- Instrument Specialist Pharmaceutical Sciences Department, University of Kentucky Feb. 2005- July 2008 Maintained and upgraded NMR instrument Trained new users and troubleshooted problems
- Brookhaven National Laboratory Brookhaven, NY RapiData: Rapid Data Collection and Structure Solving at the NSLS: A Practical Course in Macromolecular X-ray Diffraction Measurement. (Experimenter)
- Research Assistant Pharmaceutical Sciences Department, University of Kentucky June 2003- August 2008 Jurgen Rohr, Ph.D., Principle Investigator Specialized in the cloning, overexpression, and purification of proteins isolated from natural product biosynthetic pathways Performed site directed mutagenesis of important amino acids using PCR Evaluated purity of structures using HPLC and LCMS Performed enzyme kinetics experiments to determine rate constants to evaluate drug efficacy Utilized NMR and X-ray crystallography for structural analysis Performed bioactivity experiments using SRB assay
- Undergraduate Research Assistantships Pharmaceutical Sciences Department, University of Kentucky Summer 2002 Performed liquid phase peptide synthesis and characterization of peptides Kyung Bo Kim, Ph.D., Principle Investigator Chemistry Department, Berea College Summer 2001 Performed solid phase peptide synthesis Matthew Saderholm, Ph.D., Principle Investigator
- Teaching Assistant Chemistry Department, Berea College Sep. 2002-May 2003 Organic Chemistry lab instructor
- Editor, Chimes Yearbook, Berea College 2001-2002 Lead staff in completion of yearbook design including organization of photography and copy editing
Society Memberships
- American Chemical Society
- Rho Chi Honor Society
- Amercian Association for the Advancement of Science
- American Association of Pharmaceutical Scientists
Academic and Professional Honors
- 2006 30th National Medicinal Chemistry Symposium Travel Award
- 2005-06 Peter G Glavinos, Jr. Ph.D. Fall Graduate Student Travel Award
Presentations
- Varying the substrate specificity of the Baeyer-Villiger type monooxygenase mtmOIV using site directed mutagenesis to produce superior analogs of the anticancer agent Mithramycin. Miranda Beam, Marie Wehenkel and Jurgen Rohr, University of Kentucky. (oral) CERMACS Cincinnati, OH 2007.
- Altering substrate specificity of the Baeyer-Villiger type monooxygenase MtmOIV by site directed mutagenesis. Miranda Beam, Matthew McConnell, Marcos Oliveira, and Jurgen Rohr. 30th National Medicinal Chemistry Symposium. (poster) Seattle, WA. 2006.
- Structural characterization of the isolated natural product Baeyer-Villiger type oxygenase MtmOIV from the anticancer drug Mithramycin biosynthetic pathway. Miranda Gibson, Chenchen Wang, Marcos Oliveira, and Jurgen Rohr. AAPS Annual Meeting and Exposition. (poster) Nashville, TN. 2005.
- MtmOIV, a key Baeyer-Villiger type oxygenase of the Mithramycin biosynthetic pathway. Miranda Gibson, Md. Nur-e-alam, Chenchen Wang, Jurgen Rohr. 229th American Chemical Society National Meeting, Medicinal Chemistry Division. (poster) San Diego, CA. 2005.
Publications
- Miranda Beam, Mary Bosserman, Nicholas Noinaj, Marie Wehenkel, Jurgen Rohr. "Crystal structure of MtmOIV, a Baeyer-Villiger monooxygenase from the biosynthetic pathway of the anticancer agent mithramycin." Biochemistry, submitted.
- Miranda Gibson, Mohammad Nur-e-alam, Fredilyn Lipata, Marcos A. Oliveira, Jurgen Rohr. "Characterization of Kinetics and Products of the Baeyer-Villiger Oxygenase MtmOIV, the Key Enzyme of the Biosynthetic Pathway Towards the Natural Product Anticancer Drug Mithramycin from Streptomyces argillaceus." Journal of the American Chemical Society, 127(50); 17594-17595, 2005.
- Chenchen Wang, Miranda Gibson, Jurgen Rohr, and Marcos A. Oliveira. "Crystallization and X-ray diffraction properties of Baeyer-Villiger monooxygenase MtmOIV from the mithramycin biosynthetic pathway in Streptomyces Argillaceus." Acta Cryst, F61 1023-1026, 2005.
- Andriy Luzhetskyy, Lili Zhu, Miranda Gibson, Marta Fedoryshyn, Clemens Durr, Carsten Hofmann, Dirk Hoffmeister, Bohdan Ostash, Cynthia Mattingly, Val Adams, Victor Fedorenko, Jurgen Rohr, and Andreas Bechthold. "Generation of Novel Landomycins M and O through Targeted Gene Disruption." ChemBioChem, 6, 675-678, 2005.
- Maria Perez, Felipe Lombo, Lili Zhu, Miranda Gibson, Alfredo F. Brana, Jurgen Rohr, Jose A. Salas. "Combining Sugar Biosynthesis Genes for the Generation of L- and D- amicetose and formation of two novel antitumor tetracenomycins." Chem. Commun. 1604-1606, 2005.
- Felipe Lombo, Miranda Gibson, Lisa Greenwell, Alfredo F. Brana, Jurgen Rohr, Jose A. Salas, and Carmen Mendez. "Engineering Biosynthetic Pathways for Deoxysugars: Branched-Chain Sugar Pathways and Derivatives from the Antitumor Tetracenomycin." Chemistry & Biology, 11, 1709–1718, December, 2004.
Service
- Kentucky American Water Science Fair Judge, 2006-2008
- Kentucky Science and Engineering Fair Judge, 2007
- TRY-IT program mentor and volunteer, 2007
- Student Representative for faculty search committee, 2006-2007
- Winged-Women, Inc. Volunteer, 2006
- Girls in Science program Mentor and Volunteer, 2004-2005
- Glendover Elementary Science Fair Judge, 2005